Journal: Molecular Medicine Reports

Article Title: Overexpression of caudal-related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice

doi: 10.3892/mmr.2015.3838

Figure Lengend Snippet: Identification of the pEGFP-C1-CDX2 eukaryotic expression vector and overexpression of CDX2 in the LoVo cells. (A) pEGFP-C1-CDX2 was identified using a double enzyme digestion assay with Hind II/ Bam HI. The DNA fragments of 1,055 bp (CDX2) and 4.7 kb (pEGFP-C1) were amplified. (B) Monoclones of the pEGFP-C1-CDX2 cells were visualized using light microscopy (left) and fluorescence microscopy (right). Magnification, ×100. (C) Protein expression of CDX2 was detected using western blotting. The expression of β-actin was used as internal control. M, marker; 1, double enzyme digestion; CDX2, caudal-related homeobox transcription factor 2.

Article Snippet: PCR amplifications were performed in duplicate and the conditions were as follows: Initial denaturation at 94°C for 2 min, then 35 cycles of 94°C for 30 sec, 62°C for 30 sec and 72°C for 30 sec, followed by an extension step at 72°C for 10 min. Full-length CDX2 cDNA (1,055 bp) was then cloned into the Hind II/ Bam HI sites of the pEGFP-C1 eukaryotic expression vector, (Clontech Laboratories, Inc., Mountain View, CA, USA).

Techniques: Expressing, Plasmid Preparation, Over Expression, Amplification, Light Microscopy, Fluorescence, Microscopy, Western Blot, Marker

Journal: Molecular Medicine Reports

Article Title: Overexpression of caudal-related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice

doi: 10.3892/mmr.2015.3838

Figure Lengend Snippet: Overexpression of the CDX2 gene inhibits tumour growth of LoVo cells in vivo . (A) Representative images of the tumors isolated from three mice 3 weeks after inoculation with either untreated cells, pEGFP-C1 cells or pEGFP-C1-CDX2 cells (n=3/group). (B) Overexpression of CDX2 inhibited tumour growth of the LoVo cells in vivo (n=6/group). Data are expressed as the mean ± standard error of the mean. * P<0.05 and ** P<0.01 vs. untreated and pEGFP-C1 cell groups. CDX2, caudal-related homeobox transcription factor 2.

Article Snippet: PCR amplifications were performed in duplicate and the conditions were as follows: Initial denaturation at 94°C for 2 min, then 35 cycles of 94°C for 30 sec, 62°C for 30 sec and 72°C for 30 sec, followed by an extension step at 72°C for 10 min. Full-length CDX2 cDNA (1,055 bp) was then cloned into the Hind II/ Bam HI sites of the pEGFP-C1 eukaryotic expression vector, (Clontech Laboratories, Inc., Mountain View, CA, USA).

Techniques: Over Expression, In Vivo, Isolation

Journal: Molecular Medicine Reports

Article Title: Overexpression of caudal-related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice

doi: 10.3892/mmr.2015.3838

Figure Lengend Snippet: Tumor parameters following the 10-day period prior to sacrifice of the nude mice.

Article Snippet: PCR amplifications were performed in duplicate and the conditions were as follows: Initial denaturation at 94°C for 2 min, then 35 cycles of 94°C for 30 sec, 62°C for 30 sec and 72°C for 30 sec, followed by an extension step at 72°C for 10 min. Full-length CDX2 cDNA (1,055 bp) was then cloned into the Hind II/ Bam HI sites of the pEGFP-C1 eukaryotic expression vector, (Clontech Laboratories, Inc., Mountain View, CA, USA).

Techniques: Inhibition